Since the range of values displayed in a digital image normally starts at zero (0), in order to have 64 values, the maximum value possible would be 63.
Direct testing of the fossil material itself or materials associated with it, and indirect testing of material in stratigraphic contexts that bookend the fossil (i.e.
This illustration shows two hands juggle three balls representing radiometric, spectral and spatial resolution.This collage shows the difference in the level of detail between the two representations.Imagery data are represented by positive digital numbers which vary from 0 to (one less than) a selected power of 2. The maximum number of brightness levels available depends on the number of bits used in representing the energy recorded.Thus, the radiometric resolution would be much less.Image data are generally displayed in a range of grey tones, with black representing a digital number of 0 and white representing the maximum value (for example, 255 in 8-bit data).All other bleaching methods are derived from this basic principle (Figure 1).(green fluorescent protein cloned from the jellyfish A.Fluorescence Localization After Photobleaching (FLAP) is a ratiometric method which can be applied to two channels.This range corresponds to the number of bits used for coding numbers in binary format. Thus, if a sensor used 8 bits to record the data, there would be 28=256 digital values available, ranging from 0 to 255.However, if only 4 bits were used, then only 24=16 values ranging from 0 to 15 would be available.Photobleaching experiments can be conducted with confocal laser scanning microscopes where the laser is used at high intensity for bleaching and low intensity for image recording.The most common way to fluorescently tag proteins in living cells is mutants EGFP is the best because of its high quantum yield, its low tendency of photobleaching as well as its relative photostability during post-bleach image acquisition.